EasyFuge - Disposable centrifuge insert for cell attachment

Fluorescence microscopy of Buccal cells attached to a slide using EasyFuge

EasyFuge is a simple plastic adaptor for use with any standard benchtop centrifuge that enables liquid-suspended cells and particles to be attached to glass for microscopical imaging. It eliminates the need for expensive specialist hardware and, besides being easy to use, EasyFuge’s cell attachment is efficient and allows handling of low volume samples.

Features   Benefits
Suitable for low volume samples (<50 µl) Effective cell transfer with minimal sample waste
Compatible with standard centrifuges No requirement for expensive specialist equipment
Simple design and constructions Quick easy to use
Disposable device Reduced risk of contamination, and no requirement for cleaning, sterilization or maintenance

The Challenge

Currently, attaching liquid-suspended cells to a microscope slide or coverslip requires expensive, specialised centrifuge instruments. As well as being costly, the process is time consuming, and the equipment requires regular cleaning and maintenance as re-use of attachments needed for these centrifuges can also lead to sample contamination.

Technology

Edinburgh’s EasyFuge technology comprises a small, disposable, plastic centrifuge insert into which suspended cells of interest are placed. Cells can then simply and efficiently transferred onto glass using a standard centrifuge, commonly available in most research laboratories. Cleaning and contamination issues are avoided with the low cost, disposable adaptor. This innovative design is suitable for volume samples between 25-150 µ and performs particularly well for 25-50 µ volumes.

Exemplification Data

Direct comparisons of 3D-printed EasyFuge prototypes have been shown to be as effective at attaching cells to glass as a specialised centrifuge instrument. The EasyFuge devices have been tested using cultured Bone Marrow Macrophage (BMM) cells, fresh primary Buccal Cavity (BC) cells and Jurkat cells at different concentrations and volumes.

Applications

  • Cytology
  • Virology
  • Oncology
  • Microbiology

IP Status

Patent application filed

Development Status

Working Prototype

Commercial Offering

The University of Edinburgh is seeking commercial partners to license this technology and/or collaborate on co-development of the technology for commercial use.

Register your interest

If you or your company would like to find out more about this technology opportunity under Confidentiality Agreement with a view to a licence or collaborative research agreement, please complete and submit our technology enquiry form.

View technology enquiry form


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